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92
ATCC hrv b14 infectious cdna pwr3 26 vrmc 7
Hrv B14 Infectious Cdna Pwr3 26 Vrmc 7, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
ATCC human rhinovirus b14 hrv b14
Acid sensitivity of Enterovirus-D68 strains. (A) Hela cells were pretreated with 200 nM bafilomycin A1 (BAF-A1) for 1 h at 37 °C followed by infection with Enterovirus-D68 (EV-D68) strains at multiplicity of infection (MOI) 1. After 24 h, virus titers in supernatants were determined by end point dilution. Data displayed represents averages of technical replicates ± standard error of the mean (SEM) and are derived from three independent experiments. Statistical analysis was performed with Students t test comparing untreated to BAF-A1 treated infected cells. P > 0.05; * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; **** P ≤ 0.0001 (B) Hela cells were pretreated with 200 nM BAF-A1 for 1h at 37 °C followed by infection with EV-D68 strains at MOI 1. After 24 h, cells were fixed and stained for the presence of double stranded RNA (green) and nuclei were visualized using Hoechst (blue). (C) Quantification of infection percentage comparing EV-D68 and human <t>rhinovirus-B14</t> (RV-B14) infected Hela cells with untreated or BAF-A1 pretreatment. Data displayed represents averages of technical replicates ± SEM and are derived from three independent experiments. (D) Quantification of infection percentage comparing EV-D68 infected Hela cells at MOI 1 with untreated or BAF-A1 pretreatment 6 h post infection. Data displayed represents averages of technical replicates ± SEM and are derived from three independent experiments.
Human Rhinovirus B14 Hrv B14, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
human rhinovirus b14 hrv b14 - by Bioz Stars, 2026-05
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Acid sensitivity of Enterovirus-D68 strains. (A) Hela cells were pretreated with 200 nM bafilomycin A1 (BAF-A1) for 1 h at 37 °C followed by infection with Enterovirus-D68 (EV-D68) strains at multiplicity of infection (MOI) 1. After 24 h, virus titers in supernatants were determined by end point dilution. Data displayed represents averages of technical replicates ± standard error of the mean (SEM) and are derived from three independent experiments. Statistical analysis was performed with Students t test comparing untreated to BAF-A1 treated infected cells. P > 0.05; * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; **** P ≤ 0.0001 (B) Hela cells were pretreated with 200 nM BAF-A1 for 1h at 37 °C followed by infection with EV-D68 strains at MOI 1. After 24 h, cells were fixed and stained for the presence of double stranded RNA (green) and nuclei were visualized using Hoechst (blue). (C) Quantification of infection percentage comparing EV-D68 and human rhinovirus-B14 (RV-B14) infected Hela cells with untreated or BAF-A1 pretreatment. Data displayed represents averages of technical replicates ± SEM and are derived from three independent experiments. (D) Quantification of infection percentage comparing EV-D68 infected Hela cells at MOI 1 with untreated or BAF-A1 pretreatment 6 h post infection. Data displayed represents averages of technical replicates ± SEM and are derived from three independent experiments.

Journal: ACS Infectious Diseases

Article Title: Sialic Acid-Containing Glycolipids Extend the Receptor Repertoire of Enterovirus-D68

doi: 10.1021/acsinfecdis.5c00063

Figure Lengend Snippet: Acid sensitivity of Enterovirus-D68 strains. (A) Hela cells were pretreated with 200 nM bafilomycin A1 (BAF-A1) for 1 h at 37 °C followed by infection with Enterovirus-D68 (EV-D68) strains at multiplicity of infection (MOI) 1. After 24 h, virus titers in supernatants were determined by end point dilution. Data displayed represents averages of technical replicates ± standard error of the mean (SEM) and are derived from three independent experiments. Statistical analysis was performed with Students t test comparing untreated to BAF-A1 treated infected cells. P > 0.05; * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001; **** P ≤ 0.0001 (B) Hela cells were pretreated with 200 nM BAF-A1 for 1h at 37 °C followed by infection with EV-D68 strains at MOI 1. After 24 h, cells were fixed and stained for the presence of double stranded RNA (green) and nuclei were visualized using Hoechst (blue). (C) Quantification of infection percentage comparing EV-D68 and human rhinovirus-B14 (RV-B14) infected Hela cells with untreated or BAF-A1 pretreatment. Data displayed represents averages of technical replicates ± SEM and are derived from three independent experiments. (D) Quantification of infection percentage comparing EV-D68 infected Hela cells at MOI 1 with untreated or BAF-A1 pretreatment 6 h post infection. Data displayed represents averages of technical replicates ± SEM and are derived from three independent experiments.

Article Snippet: Infectious clone of human rhinovirus B14 (HRV-B14) (pWR3.26) was purchased from ATCC.

Techniques: Infection, Virus, Derivative Assay, Staining